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Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
 

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Article DOI: 10.3791/59043-v 10:39 min April 16th, 2019
April 16th, 2019

챕터

요약

The here introduced protocol allows characterization of the lung homing capacity of primary human lymphocytes under in vivo inflammatory conditions. Pulmonary infiltration of adoptively transferred human immune cells in a mouse model of allergic inflammation can be imaged and quantified by light-sheet fluorescence microscopy of chemically cleared lung tissue.

Tags

Keywords: Lung Homing Human Lymphocytes Allergic Inflammation Light-sheet Microscopy T Cell Lung Homing Inflammatory Lung Diseases Papain Ficoll PBMC CD4+ T Cells Cell Proliferation Dye Adoptive Transfer
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