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Chapters
- 00:04Title
- 00:25Protein Crystal Sample Preparation
- 03:00Testing Sample Extrusion Using a High-Speed Camera Setup
- 05:43Results: Extrusion of Protein Microcrystals from a High Viscosity Micro-extrusion Injector
- 07:03Conclusion
The success of a time-resolved serial femtosecond crystallography experiment is dependent on efficient sample delivery. Here, we describe protocols to optimize the extrusion of bacteriorhodopsin microcrystals from a high viscosity micro-extrusion injector. The methodology relies on sample homogenization with a novel three-way coupler and visualization with a high-speed camera.
