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改进 x 射线激光时分辨序列飞秒晶体学微晶的高粘度挤出

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Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

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改进 x 射线激光时分辨序列飞秒晶体学微晶的高粘度挤出

DOI:

10.3791/59087-v

•

07:26 min

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February 28, 2019

•

Daniel James, Tobias Weinert, Petr Skopintsev, Antonia Furrer, Dardan Gashi², Tomoyuki Tanaka⁴, Eriko Nango⁴, Przemyslaw Nogly⁵, Joerg Standfuss

¹Division of Biology and Chemistry – Laboratory for Biomolecular Research,Paul Scherrer Institut, ²Photon Science Division – SwissFEL,Paul Scherrer Institut, ³RIKEN SPring-8 Center, ⁴Department of Cell Biology, Graduate School of Medicine,Kyoto University, ⁵Department of Biology,ETH Zürich

Chapters

00:04Title
00:25Protein Crystal Sample Preparation
03:00Testing Sample Extrusion Using a High-Speed Camera Setup
05:43Results: Extrusion of Protein Microcrystals from a High Viscosity Micro-extrusion Injector
07:03Conclusion

Summary

자동 번역

时间解析的飞秒晶体学实验的成功取决于有效的样品传递。在这里, 我们描述了从高粘度微挤压喷射器中优化细菌视紫红质微晶挤出的方案。该方法依赖于样品同质化与一种新的三向耦合器和可视化与高速相机。

Tags

Keywords: High Viscosity Extrusion Microcrystals Time-resolved Serial Femtosecond Crystallography X-ray Lasers LCP Monoolein MAG 7.9 Liquid Paraffin Syringe Cubic Phase Extrusion Testing HPLC Pump

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