Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolation and Adoptive Transfer of High Salt…
JoVE Journal
Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Immunology and Infection
Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
 

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Article DOI: 10.3791/59124-v • 09:29 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1, 1, 1
1Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center

챕터

요약
Automatic Translation

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

Tags

Keywords: Dendritic Cells CD11c Hypertension Cardiovascular Disease Adoptive Transfer Spleen Isolation Collagenase D DNase MACS Flow Cytometry
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter