Journal
/
생화학
/
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

DOI:
10.3791/59152-v

•

09:37 min

•

February 12, 2019

•

, , , , ,
1Department of Chemistry,Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Chapters

  • 00:04Title
  • 01:2313C, 15N-Labeled Aspergillus fumigatus Liquid and Growth Media Preparation
  • 03:03Fungal Materials Growth
  • 04:21A. fumigatus Preparation for Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP) Studies
  • 06:47Plant Biomass Preparation for DNP Analysis
  • 07:49Results: Fungal Cell Wall Structure Characterization by Solid-State NMR
  • 09:02Conclusion

Summary

자동 번역

자동 번역

A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.

Tags

Fungal MaterialsPlant MaterialsSolid-state NMRDynamic Nuclear PolarizationBiomaterialsCarbohydratesFungal Cell WallsCell CultureYPD MediumLabeled Minimal MediumIsotope LabelingCarbon-13Nitrogen-15SterilizationAutoclaveCell Culture Techniques

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/59152?article_type=v

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
check_url/kr/59152?article_type=v

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
check_url/kr/59152?article_type=v

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
check_url/kr/59152?article_type=v

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
check_url/kr/59152?article_type=v

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
check_url/kr/59152?article_type=v

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
check_url/kr/59152?article_type=v

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
check_url/kr/59152?article_type=v

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
check_url/kr/59152?article_type=v

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
check_url/kr/59152?article_type=v

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Read Article