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中通量筛选法评估对精子中 ca2 +信号和反应的影响
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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
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中通量筛选法评估对精子中 ca2 +信号和反应的影响

DOI:
10.3791/59212-v

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05:44 min

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March 01, 2019

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, , , ,
1Department of Growth and Reproduction,Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC),University of Copenhagen

Chapters

  • 00:04Title
  • 00:40Motile Sperm Cell Purification
  • 01:28Sperm Cell Counting
  • 02:14Calcium-fluorimetry
  • 03:30Acrosome Reaction Measurement
  • 04:14Results: Representative Calcium-signaling and Acrosome Reaction Analyses
  • 05:01Conclusion

Summary

자동 번역

자동 번역

在这里, 描述了两个中等吞吐量检测评估对 ca2+信号和顶体反应的影响在人类精子中。这些检测方法可用于快速、轻松地筛选大量化合物, 以确定对人类精子中 ca2 +信号和顶体反应的影响。

Tags

Medium-throughput ScreeningCalcium SignalingAcrosome ReactionHuman SpermSperm Cell FunctionCompound TestingHTF MediumSperm Cell MotilitySperm Cell ConcentrationFluorescence Plate ReaderCalcium Concentration

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