Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Generation, Amplification, and Titration of…
JoVE Journal
Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Immunology and Infection
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
 

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Article DOI: 10.3791/59218-v • 11:48 min • April 4th, 2019
April 4th, 2019 •
1, 1, 1, 1, 1, 2, 1,3, 1,3
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

챕터

요약
Automatic Translation

We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.

Tags

Keywords: Recombinant Respiratory Syncytial Virus Virus Rescue Virus Amplification Virus Titration BSR-T7/5 Cells Transfection HEp-2 Cells Virus Stock Generation Virological Studies
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter