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A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
 

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

Article DOI: 10.3791/59295-v • 04:19 min • April 19th, 2019
April 19th, 2019 •
*1,2,3, *1,2,3, 1,2,3, 3,4, 3,4, 1,2,3
1Bayer School of Natural and Environmental Sciences, Duquesne University, 2Department of Biological Sciences, Duquesne University, 3Chronic Pain Research Consortium, Duquesne University, 4Graduate School of Pharmaceutical Sciences, Duquesne University
* These authors contributed equally

챕터

요약
Automatic Translation

Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

Tags

Keywords: Tail Vein Injection Near-infrared-labeled Agents Fluorescence Imaging Anesthetized Animal Tail Vasculature Lateral Tail Vein 25-27 Gauge Needle Needle Placement Syringe Injection Catheter Blood Flashback Quality Control Animal Studies Reproducibility
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