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Developmental Biology

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JoVE Journal Developmental Biology
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
 

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Article DOI: 10.3791/59353-v • 12:34 min • May 9th, 2019
May 9th, 2019 •
1, 1
1Department of Dermatology and Charles C. Gates Center for Regenerative Medicine, University of Colorado Anschutz Medical Campus

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요약
Automatic Translation

This protocol describes how to isolate skin keratinocytes from mouse models, to stain with metal-tagged antibodies, and to analyze stained cells by mass cytometry in order to profile the expression pattern of proteins of interest in the different cell cycle phases.

Tags

Keywords: Keratinocytes Cell Cycle Mass Cytometry Protein Expression Cell Proliferation Cancer Research Cell Isolation Skin Cells IdU Paraformaldehyde Cisplatin
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