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Immunology and Infection
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JoVE Journal Immunology and Infection
Design af cecal ligation og punktering og intranasal infektion Dual model af sepsis-induceret immunsuppression
 
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Design af cecal ligation og punktering og intranasal infektion Dual model af sepsis-induceret immunsuppression

Article DOI: 10.3791/59386-v • 07:30 min • June 15th, 2019
June 15th, 2019 •
*1,2, *1,2, *2,3, 1, 3, 2
1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University
* These authors contributed equally

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Denne protokol beskriver teknikker til at måle infektiøse resultater underliggende sekundære hospitalserhvervede infektioner i immunsuppressiv tilstand, først ved at etablere cecal ligation/punktering mus derefter udfordre dem med intranasal infektion til at skabe en klinisk relevant model for immun undertrykkelses sepsis.

Tags

Immunologi og infektion cecal ligation og punktering intranasal infektion sepsis-induceret immunsuppression immunsuppression sepsis sepsis Staphylococcus aureus nosokomielle pneumonias mus Dual model dobbelt-hit model
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