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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Chapters
- 00:04Title
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fisiológicamente, los receptores odorantes son activados por moléculas odorantes inhaladas en la fase de vapor. Sin embargo, más sistemas in vitro utilizan la estimulación olfativa de fase líquida. Aquí, presentamos un método que permite en tiempo real monitoreo in vitro de la activación del receptor odorante al estímulo odorante en fase de vapor.
