Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

Meng Zhao; Yawen Tang; Patrick  J. Ernst; Asher Kahn-Krell; Chengming Fan; Danielle Pretorius; Hanxi Zhu; Xi Lou; Lufang Zhou; Jianyi Zhang; Wuqiang Zhu

doi:10.3791/59452

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Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

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Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

DOI:

10.3791/59452-v

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08:00 min

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July 10, 2019

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Meng Zhao, Yawen Tang, Patrick J. Ernst², Asher Kahn-Krell, Chengming Fan³, Danielle Pretorius, Hanxi Zhu, Xi Lou, Lufang Zhou, Jianyi Zhang, Wuqiang Zhu

¹Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham, ²Division of Cardiovascular Diseases, School of Medicine, School of Engineering,University of Alabama at Birmingham, ³Department of Cardiac Surgery,The Second Xiangya Hospital of Central South University

Chapters

00:04Title
01:13hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
03:42Calcium Transient and Contractility Recording
05:21Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
07:11Conclusion

Summary

자동 번역

In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

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