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Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
 

Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

Article DOI: 10.3791/59484-v 07:38 min May 17th, 2019
May 17th, 2019

챕터

요약

Using three-dimensional organotypic cultures to visualize morphology and functional markers of salivary glands may provide novel insights into the mechanisms of tissue damage following radiation. Described here is a protocol to section, culture, irradiate, stain, and image 50–90 μm thick salivary gland sections prior to and following exposure to ionizing radiation.

Tags

Keywords: Organotypic Culture Submandibular Salivary Gland Parotid Salivary Gland Radiation Treatment Ex Vivo Tissue Sectioning Vibratome Agarose Embedding Cell-cell Interactions Therapeutic Screening Salivary Gland Damage Radiotherapy Gene Manipulation
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