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JoVE Journal Bioengineering
In vivo tweekleurige 2-Fobe beeldvorming van genetisch gelabelde reporter cellen in de huid
 
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In vivo tweekleurige 2-Fobe beeldvorming van genetisch gelabelde reporter cellen in de huid

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Morfologische veranderingen optreden in immuun responsieve fibroblast cellen na activering en het bevorderen van veranderingen in cellulaire werving. Gebruik makend van 2-Photon Imaging in combinatie met een genetisch gemanipuleerde fibroblast-specifieke proteïne 1 (FSP1)-CRE; tdTomato floxed-stop-floxed (TB/TB) muis lijn en groene fluorescently gelabeld lipopolysaccharide-FITC, we kunnen zeer specifieke opname van lipopolysaccharide in dermale fibroblasten en morfologische veranderingen in vivo illustreren.

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Biotechniek uitgave 149 multi-Photon Imaging tol-achtige receptor 4 genetische reporter lipopolysaccharide in vivo
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