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Chapters
- 00:04Title
- 01:04Setting Up the Photostimulation System
- 01:48Preparing for Activation of ERK2 by Photostimulation
- 02:47Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-A Mode
- 04:24Delivering Femtosecond Laser Stimulation into Target Cells Using Stim-B Mode
- 05:53Activation of Mitoflashes and Other Mitochondrial Events by Photostimulation
- 06:55Delivering Femtosecond Laser Stimulation into the Target Mitochondrion Using Stim-A Mode
- 08:32Results: Activation of ERK Signaling or Mitochondrial Events in Target Cells
- 10:26Conclusion
Tightly-focused femtosecond laser can deliver precise stimulation to cells by being coupled into a confocal microscopy enabling the real-time observation and photostimulation. The photostimulation can activate cell molecular events including ERK signaling pathway and mitochondrial flashes of reactive oxygen species.
