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Developmental Biology

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JoVE Journal Developmental Biology
Snelle en efficiënte expressie van meervoudige eiwitten in aviaire embryo's met behulp van mRNA-Elektroporation
 
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Snelle en efficiënte expressie van meervoudige eiwitten in aviaire embryo's met behulp van mRNA-Elektroporation

Article DOI: 10.3791/59664-v • 09:40 min • June 7th, 2019
June 7th, 2019 •
1, 2,3, 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

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We rapporteren boodschapper RNA (mRNA) electroporatie als een methode die snelle en efficiënte expressie van meerdere eiwitten in het kwartel embryo modelsysteem toestaat. Deze methode kan worden gebruikt om cellen fluorescentend te labelen en hun in vivo bewegingen op te nemen door middel van timelapse-microscopie kort na elektroporation.

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Ontwikkelingsbiologie uitgave 148 mRNA electroporation time-lapse microscopie kwartel aviaire embryo FRAP
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