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Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
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Chapters
- 00:04Title
- 01:15Ligature Installation
- 03:29Sample Collection
- 05:05Antibody Staining for Flow Cytometric Analysis
- 06:03Results: FACS and Alveolar Bone Loss Analyses
- 06:37Conclusion
This article presents a protocol for establishing a ligature-induced model of murine periodontitis involving multiple maxillary molars, resulting in larger areas of the involved gingival tissue and bone for subsequent analysis as well as reduced animal usage. A technique to assess oral neutrophils in a manner analogous to human subjects is also described.
