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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
JoVE 신문
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JoVE 신문 암 연구학
Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
DOI:

05:17 min

May 14, 2019

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Chapters

  • 00:04Title
  • 00:28Circulating Tumor Cell (CTC) Micromanipulation and Single-Cell Picking
  • 03:31Results: Representative Downstream Molecular Analysis and Metastatic Potential Assessment of Single-Cell Picked CTC
  • 04:42Conclusion

Summary

자동 번역

Here, we present an integrated workflow to identify phenotypic and molecular features that characterize circulating tumor cells (CTCs). We combine live immunostaining and robotic micromanipulation of single and clustered CTCs with single cell-based techniques for downstream analysis and assessment of metastasis-seeding ability.

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