Journal
/
암 연구학
/
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

DOI:
10.3791/59679-v

•

08:17 min

•

August 15, 2019

•

, , , , , , , , , , , , , ,
1BeiGene (Beijing) Co., Ltd.

Chapters

  • 00:04Title
  • 00:46Cyclophosphamide Myeloablation and Human Peripheral Blood Mononuclear Cell (PBMC) Transplantation and Tumor Engraftment
  • 02:08Immunohistochemistry (IHC)
  • 04:33In Vivo Efficacy and Pharmacodynamics Analyses
  • 06:08Results: Representative PBMC- and Tumor-Engraftment and Anti-Tumor Antibody Treatment Analysis
  • 07:16Conclusion

Summary

자동 번역

자동 번역

We describe a human peripheral blood mononuclear cell (PBMC) — based humanized xenograft mouse model for translational immuno-oncology research. This protocol could serve as a general guideline for establishing and characterizing similar models for I-O therapy assessment.

Tags

PBMCHumanized XenograftImmuno-oncologyMyeloid Cell DepletionCyclophosphamideDisulfiramTumor GrowthImmunohistochemistryAntigen Retrieval

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/59679?article_type=v

A Mouse Model of Fatigue Induced by Peripheral Irradiation
check_url/kr/59679?article_type=v

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
check_url/kr/59679?article_type=v

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
check_url/kr/59679?article_type=v

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
check_url/kr/59679?article_type=v

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
check_url/kr/59679?article_type=v

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
check_url/kr/59679?article_type=v

A Melanoma Patient-Derived Xenograft Model
check_url/kr/59679?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/kr/59679?article_type=v

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
check_url/kr/59679?article_type=v

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Read Article