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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
 

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Article DOI: 10.3791/59706-v • 09:57 min • August 12th, 2019
August 12th, 2019 •
1, 1, 1,2
1Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, 2Department of Biochemistry, Wake Forest School of Medicine

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요약
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Here we present a protocol to quantify the chemotactic activity of blood monocytes in mouse models, to assess the effects of nutritional, pharmacological and genetic interventions on blood monocyte and to characterize the blood monocytes derived macrophages in mouse models using monocyte-chemoattractant protein-1 (MCP-1)-loaded basement membrane-derived gel plugs.

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Quantification Monocyte Chemotactic Activity In Vivo Blood Monocyte Derived Macrophages Poor Diets Monocyte Function Underlying Mechanisms Isolation Analysis Single Cell Techniques Omics Techniques Health Mouse Models Procedure Dr. Young Joo Ahn Assistant Professor Dr. Luxi Wang Postdoc Cell Culture Hood Disinfectant Growth Factor-reduced Basement Membrane-derived Solution Ice Syringes Needles Chemoattractant Air Bubbles Plug Formation Response To Toe Pinch
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