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DNA-sequentie herkenning door DNA primase met behulp van high-throughput Primase profilering
JoVE 신문
생화학
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JoVE 신문 생화학
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
DOI:

08:04 min

October 08, 2019

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Chapters

  • 00:04Title
  • 01:29Primase DNA Binding Experiment: Blocking Procedure
  • 02:44Primase DNA Binding Experiment: Protein Binding
  • 03:22Primase DNA Binding Experiment: Fluorescent Antibody Attachment
  • 05:15Results: Analysis of High-Throughput Primase Profiling
  • 06:42Conclusion

Summary

자동 번역

Experimenten met eiwitbinding Microarray (PBM) in combinatie met biochemische assays koppelen de bindende en katalytische eigenschappen van DNA primase, een enzym dat RNA-primers op Template DNA synthetiseert. Deze methode, aangeduid als high-throughput Primase profilering (HTPP), kan worden gebruikt om DNA-bindende patronen van een verscheidenheid aan enzymen te onthullen.

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