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JoVE Journal Biochemistry
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
 

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Article DOI: 10.3791/59918-v • 10:27 min • July 8th, 2019
July 8th, 2019 •
1, 1, 2
1Department of Chemistry, University of Houston, 2Department of Biology and Biochemistry, University of Houston

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요약
Automatic Translation

Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.

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Keywords: Dual DNA Ruler Ribosome Translocation Single-nucleotide Resolution DNA Binding Chemotherapeutical Drugs Streptavidin Ribosome Complex Antibiotics Neomycin EFG GTP PEP Pyruvate Kinase Fusidic Acid Viomycin Hygromycin B Frame Shifting Slippery Motif U6A
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