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Biochemistry

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JoVE Journal Biochemistry
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
 

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Article DOI: 10.3791/57807-v • 09:35 min • October 15th, 2018
October 15th, 2018 •
1,2, 3, 3
1Department of Health Science, School of Sports & Health Science, Daito Bunka University, 2Department of Molecular Life Science, School of Medicine, Tokai University, 3Department of Health Management, School of Health Study, Tokai University

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요약
Automatic Translation

We describe a modified small-scale extraction and colorimetric assays of lactate and pyruvate in the nematode C. elegans. When utilizing commercial assay kits, the technical development of their sensitivity and accuracy is important. Protein precipitation in extraction is the most critical step for the quantitative determination of intracellular metabolites.

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Keywords: Colorimetric Assay Lactate Pyruvate Caenorhabditis Elegans Nematode Protein Precipitation Homogenization E. Coli OP50 NGM Agar Plates Gravid Hermaphrodites Egg Isolation L1 Larvae Synchronous Culture Colorimetric Analysis Biochemical Methodology
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