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Biochemistry

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JoVE Journal Biochemistry
Plate-based Large-scale Cultivation of Caenorhabditis elegans
 

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Article DOI: 10.3791/58117-v • 05:08 min • August 24th, 2018
August 24th, 2018 •
1, 2,3, 1, 1,4, *1, *1
15th Medical Department, Medical Faculty Mannheim, Heidelberg University, 2Department of Internal Medicine, Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.

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Keywords: Caenorhabditis Elegans Plate-based Cultivation Large-scale Cultivation Nematode Population Synchronized Population M9 Buffer Sample Preparation Metabolic Alterations Diabetes Microscope Density Evaluation Pipet Tips Shear Stress Liquid Nitrogen Centrifugation Sample Washing Snap Freezing
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