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细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定

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Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

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细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定

DOI:

10.3791/59968-v

•

08:21 min

•

September 01, 2019

•

Ramon Lopez Perez², Franziska Münz², Jonas Kroschke², Jannek Brauer², Nils H. Nicolay^2,3, Peter E. Huber²

¹CCU Molecular and Radiation Oncology,German Cancer Research Center, ²Department of Radiation Oncology,Heidelberg University Hospital, ³Department of Radiation Oncology,Freiburg University Medical Center

Chapters

00:04Title
00:56Sample Collection
01:44Cell Staining
02:45Analysis
04:28Data Evaluation
06:15Results: DNA Damage Response After Different Types of Radiation
07:24Conclusion

Summary

자동 번역

该方法结合了DNA双链断裂（DSB）、细胞循环分布和凋亡的定量分析，实现了对DSB诱导和修复以及修复失败后果的细胞周期特定评估。

Tags

Keywords: Cell Cycle DNA Damage Genotoxic Stress Flow Cytometry Î³H2AX Apoptosis Radiobiology Radiotherapy Oncology DNA Staining Cell Fixation Cell Suspension

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