Genetic Mapping of Thermotolerance Differences Between Species of <em>Saccharomyces</em> Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Carly V. Weiss; Julie N. Chuong; Rachel B. Brem

doi:10.3791/59972

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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

DOI:

10.3791/59972-v

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10:08 min

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August 12, 2019

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Carly V. Weiss², Julie N. Chuong, Rachel B. Brem³

¹Department of Plant and Microbial Biology,University of California Berkeley, ²Department of Biology,Stanford University, ³Buck Institute for Research on Aging

Chapters

00:04Title
00:22Preparation of Hybrid Yeast Cells for Transformation
01:55Transformation of pJR487 into Hybrid Yeast Cells
08:16Results: Thermotolerance Differences between Species of Saccharomyces
09:44Conclusion

Summary

자동 번역

Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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