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1Division of Nephrology and Clinical Immunology,University Hospital RWTH Aachen, 2III. Department of Medicine,University Medical Center, Hamburg-Eppendorf, 3Department of Nephrology,Monash Health, 4Division of Nephrology and Hypertension,Oregon Health and Science University, 5Renal Section,Veterans Affairs Portland Health Care System, 6Fondation LeDucq Transatlantic Networks of Excellence, 7Department of Internal Medicine, Nephrology and Transplantation,Erasmus Medical Center
Chapters
- 00:04Title
- 01:11Fixation and Immunostaining of Mouse Kidneys
- 03:26Tissue Clearing and Imaging
- 04:13Results: Immunolabeled and Optically Cleared Kidney Tissue
- 05:23Conclusion
De combinatie van antilichaam etikettering, optische clearing en geavanceerde Lichtmicroscopie maakt een driedimensionale analyse van complete structuren of organen mogelijk. Hier beschreven is een eenvoudige methode om immunolabeling van dikke nierschijfjes te combineren, optische clearing met ethyl CINNAMAAT, en confocale beeldvorming die visualisatie en kwantificering van driedimensionale nierstructuren mogelijk maakt.
