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Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
 

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Article DOI: 10.3791/60018-v 09:15 min August 16th, 2019
August 16th, 2019

챕터

요약

Here, we present detailed processing protocols for imaging delicate tissue samples using scanning electron microscopy (SEM). Three different processing methods, namely, hexamethyl disilazana (HMDS) chemical drying, simple air drying, and critical point drying are described for preparing rigid eggshells, embryos at early developmental stages, and fungal cultures respectively.

Tags

Keywords: Scanning Electron Microscopy Sample Preparation Painted Turtle Embryo Rigid Eggshell Fungal Culture Tissue Processing Embryo Collection Eggshell Dissection Fixation Dehydration Critical Point Drying Mounting Sputter Coating
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