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Biochemistry

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JoVE Journal Biochemistry
Kvantificering af protein interaktions netværk dynamik ved hjælp af Multiplexed Co-Immunopræcipitation
 
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Kvantificering af protein interaktions netværk dynamik ved hjælp af Multiplexed Co-Immunopræcipitation

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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Kvantitativ multiplex Immunoprecipitation (QMI) bruger flow cytometri til følsom påvisning af forskelle i den overflod af målrettede protein-protein interaktioner mellem to prøver. QMI kan udføres ved hjælp af en lille mængde biomateriale, kræver ikke genetisk manipulerede Tags, og kan tilpasses til alle tidligere definerede protein interaktion netværk.

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Biokemi IP-FCM proteomics protein interaktion signalering immunopræcipitation QMI
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