Journal
/
생물학
/
通过抑制Efflux泵,提高造血干细胞和造血细胞中线粒体膜电位流细胞测定的准确性
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
Please note that all translations are automatically generated. Click here for the English version.

通过抑制Efflux泵,提高造血干细胞和造血细胞中线粒体膜电位流细胞测定的准确性

DOI:
10.3791/60057-v

•

07:17 min

•

July 30, 2019

•

, ,
1Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research,Albert Einstein College of Medicine, 2Departments of Cell Biology and Stem Cell Institute,Albert Einstein College of Medicine, 3Department of Medicine,Albert Einstein College of Medicine, 4Albert Einstein Cancer Center and Diabetes Research Center,Albert Einstein College of Medicine

Chapters

  • 00:04Title
  • 00:43Bone Marrow Isolation
  • 02:22Immunostaining for Detection of Hematopoietic Stem Cells
  • 03:28TMRM Staining
  • 04:17Flow Cytometry
  • 05:38Results: Flow Cytometry Analysis of Mitochondrial Membrane Potential
  • 06:33Conclusion

Summary

자동 번역

자동 번역

异种生物排泄泵在造血干细胞和祖细胞(HSPCs)中高度活跃,并导致TMRM(线粒体膜潜在荧光染料)的挤出。在这里,我们提出了一个协议,以准确测量线粒体膜电位在HSPC与TMRM的存在,Verapamil,一个排泄泵抑制剂。

Tags

Flow CytometryMitochondrial Membrane PotentialHematopoietic Stem CellsProgenitor CellsEfflux PumpsTMRMVerapamilBone Marrow IsolationLineage CocktailCD135

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

从胎盘中的造血干细胞的分离和分析

流动小鼠减数分裂细胞的流式细胞仪净化

慢病毒介导的击倒在体外红细胞

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

流细胞测定方法测量造血干细胞和T细胞的线粒体质量及膜电位

使用细胞外通量分析仪评估小鼠造血干细胞和原始祖细胞中的细胞生物能量学

使用 体内 抑制、免疫荧光和流式细胞术对精子细胞中驱动蛋白-7 CENP-E 进行功能评估

造血干细胞和祖细胞的CRISPR/Cas9基因编辑用于基因治疗应用

Read Article