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JoVE Journal Cancer Research
Differenzierung von Mausbrustepithel-HC11- und EpH4-Zellen
 
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Differenzierung von Mausbrustepithel-HC11- und EpH4-Zellen

Article DOI: 10.3791/60147-v • 09:32 min • February 27th, 2020
February 27th, 2020 •
*1,2, *1, *1,3, 1,4, 1, 1, 2, 1
1Department of Biomedical and Molecular Sciences and Department of Pathology and Molecular Medicine, Queen's University, Kingston, 2Department of Chemical and Physical Sciences, University of Toronto, Mississauga, 3Department of Laboratory Medicine, Brampton Civic Hospital Campus, William Osler Health System, 4Latner Thoracic Surgery Research Laboratories
* These authors contributed equally

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Wir beschreiben Techniken zur Differenzierungsinduktion von zwei Brustepithellinien, HC11 und EpH4. Während beide fetales Kalbsserum, Insulin und Prolaktin benötigen, um Milchproteine zu produzieren, können sich EpH4-Zellen in der dreidimensionalen Kultur vollständig in Mammosphären differenzieren. Diese komplementären Modelle sind nützlich für Signaltransduktionsstudien der Differenzierung und Neoplasie.

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Krebsforschung Ausgabe 156 Zelldifferenzierung Brustepithelzellen Prolaktin EHS-Matrix
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