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Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
 

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

Article DOI: 10.3791/60195-v • 09:11 min • September 25th, 2019
September 25th, 2019 •
1, 2, 1, 1
1Department of Biological Sciences, Carnegie Mellon University, 2QPathology

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요약
Automatic Translation

Nanoscale imaging of clinical tissue samples can improve understanding of disease pathogenesis. Expansion pathology (ExPath) is a version of expansion microscopy (ExM), modified for compatibility with standard clinical tissue samples, to explore the nanoscale configuration of biomolecules using conventional diffraction limited microscopes.

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Keywords: Nanoscopic Imaging Expansion Pathology ExPath Tissue Sections Fluorescent Labeling Biomolecules Disease-related Cancer Brain Disease Autoimmune Disease FFPE Frozen Tissue Antigen Retrieval Hydrogel Physical Expansion Isotropic Expansion Diffraction Limit Conventional Microscopy Sample Preparation Tissue Processing
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