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人类干细胞衍生内皮细胞和GABAergic神经元的迁移、化学吸引和共培养测定
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JoVE 신문 신경과학
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
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人类干细胞衍生内皮细胞和GABAergic神经元的迁移、化学吸引和共培养测定

DOI:
10.3791/60295-v

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10:09 min

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January 23, 2020

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,
1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Chapters

  • 00:05Title
  • 01:17Preparation of Human Cells for Assay
  • 03:36Preparation of One-Well Culture Inserts
  • 04:12Long-Distance Migration Assay
  • 05:48Co-culture Migration and Chemo-Attraction Assays
  • 07:28Results: Migration and Chemo-Attraction Assays
  • 08:38Conclusion

Summary

자동 번역

자동 번역

我们提出了三个简单的体外测定-远距离迁移测定，共培养迁移测定，和化学吸引力测定-共同评估人类干细胞衍生的腹膜内皮细胞的功能及其与GABAergic内神经的相互作用。

Tags

Keyword Extraction:MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays

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