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Immunology and Infection

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JoVE Journal Immunology and Infection
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
 

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Article DOI: 10.3791/60323-v • 09:34 min • October 31st, 2019
October 31st, 2019 •
1,2, 1,3, 1,4, 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research

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요약
Automatic Translation

This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

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Keywords: Endothelial Cells Primary Human Constant Flow Pneumococcus Infection Bacterial Infection Vascular Integrity Morphogenesis Inflammation Immune Regulation Endothelial Cell Differentiation Wound Healing Tumor Genesis Angiogenesis Cell Culture HUVEC Microfluidic Pump ECGMS Medium Perfusion Set
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