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1Graduate Program in Bioengineering,University of California San Francisco and University of California Berkeley, 2Center for Bioengineering and Tissue Regeneration, Department of Surgery,University of California San Francisco, 3Department of Mechanical Engineering,University of California Berkeley, 4Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco, 5UCSF Comprehensive Cancer Center, Helen Diller Family Cancer Research Center,University of California San Francisco, 6Department of Anatomy, Department of Bioengineering and Therapeutic Sciences, and Department of Radiation Oncology,University of California San Francisco
Chapters
- 00:04Title
- 00:49Generation of Stencils for Patterning ECM Ligand
- 03:47Patterning ECM Ligand on Coverslips
- 04:46Transfer of Ligand to Polyacrylamide Gel
- 07:27Results: Geometry of Human Embryonic Stem Cell Colonies
- 09:04Conclusion
Extrazelluläre Matrixliganden können auf Polyacrylamid-Hydrogele gemustert werden, um die Kultur menschlicher embryonaler Stammzellen in geschlossenen Kolonien auf konformen Substraten zu ermöglichen. Diese Methode kann mit Traktionskraftmikroskopie und biochemischen Assays kombiniert werden, um das Zusammenspiel zwischen Gewebegeometrie, zellgenerierten Kräften und Schicksalsspezifikation zu untersuchen.
