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Neuroscience

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JoVE Journal Neuroscience
Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing
 

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Article DOI: 10.3791/60347-v • 09:49 min • December 3rd, 2019
December 3rd, 2019 •
1, 1
1Department of Neurobiology, Stanford University School of Medicine

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요약
Automatic Translation

We provide a protocol for isolation of microglia from different dissected regions of an adult mouse brain hemisphere, followed by semi-automated library preparation for deep single-cell RNA sequencing of full-length transcriptomes. This method will help to elucidate functional heterogeneity of microglia in health and disease.

Tags

Keywords: Microglia Single-cell RNA Sequencing Brain Regions Tissue Dissociation Cell Isolation Cell Separation Dounce Homogenizer Magnetic Separation RNA Extraction Library Preparation Deep Sequencing
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