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Journal / Bioengineering / Tissue-Engineered Graft for Circumferential…
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Bioengineering

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JoVE Journal Bioengineering
Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats
 

Tissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats

Article DOI: 10.3791/60349-v • 08:56 min • February 10th, 2020
February 10th, 2020 •
1, 2, 3, 1, 2, 1
1Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University, College of Medicine, 2Department of Biomedical Engineering, Inje University, Gimhae, 3Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials

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요약
Automatic Translation

Esophageal reconstruction is a challenging procedure, and development of a tissue-engineered esophagus that enables regeneration of esophageal mucosa and muscle and that can be implanted as an artificial graft is necessary. Here, we present our protocol to generate an artificial esophagus, including scaffold manufacturing, bioreactor cultivation, and various surgical techniques.

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Keywords: Tissue-engineered Graft Circumferential Esophageal Reconstruction Rat Model Two-layered Tubular Scaffold Nanofiber 3D-printing Microvascular Anastomosis Bioreactor Cultivation Adipose-derived Mesenchymal Stem Cells LIVE/DEAD Assay T-tube Placement Esophageal Repair
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