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Genetics

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JoVE Journal Genetics
Measuring Microbial Mutation Rates with the Fluctuation Assay
 

Measuring Microbial Mutation Rates with the Fluctuation Assay

Article DOI: 10.3791/60406-v • 07:44 min • November 28th, 2019
November 28th, 2019 •
1, 2,3, 3, 1, 4, 3
1School of Biological Sciences, The University of Manchester, 2School of Science, University of Waikato, 3School of Natural Sciences, The University of Manchester, 4Department of Mathematics, Université du Québec à Montréal

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Here, a protocol is presented to perform a fluctuation assay and estimate microbial mutation rate using phenotypic markers. This protocol will enable researchers to assay mutations in diverse microbes and environments, determining how genotype and ecological context affect spontaneous mutation rates.

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Measuring Microbial Mutation Rates Fluctuation Assay Mutational Estimation Environmental Context Probability Of Spontaneous Mutation Cheap And Efficient Protocol Parallel Estimates Of Mutation Rate Antimicrobial Resistance Ecological Context Evolution Of Antimicrobial Resistance Monoculture Laboratory Strain Clinical Strains Co-culture Bioneutral Molecule Hazardous Reactions Antibiotic Rifampicin Methanol Protective Gloves And Goggles Liquid Lysogeny Broth E.coli K12 Glycerol Stock LB Culture Saline Solution Davis Minimal Medium
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