JoVE Journal
Cancer Research
Cancer Research
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.