Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Studying the Effects of Tumor-Secreted Paracrine…
JoVE Journal
Cancer Research

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Cancer Research
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
 

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Article DOI: 10.3791/60453-v • 07:44 min • November 28th, 2019
November 28th, 2019 •
1, 1, 2
1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 2O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham

챕터

요약
Automatic Translation

Here, we present a method using permeable membrane supports to facilitate the study of non-contact paracrine signaling used by tumor cells to suppress the immune response. The system is amenable to studying the role of tumor-secreted factors in dampening macrophage activation.

Tags

Tumor-secreted Paracrine Ligands Macrophage Activation Co-culture Permeable Membrane Supports Immune Response Suppression Secreted Factors Pro-inflammatory Gene Transcription Tumor-derived Immune Suppression Paracrine Signaling Cell-to-cell Contact Molecular Biological Techniques Peritoneal Macrophages Polyester Membrane Insert Co-culture Six-well Plate Supplemented DMEM Commercial Tumor Cells ATCC Recommended Tissue Culture Methods PBS Trypsin EDTA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter