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流细胞测定方法测量造血干细胞和T细胞的线粒体质量及膜电位

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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

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流细胞测定方法测量造血干细胞和T细胞的线粒体质量及膜电位

DOI:

10.3791/60475-v

•

07:57 min

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December 26, 2019

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Mukul Girotra*^1,2, Anne-Christine Thierry, Alexandre Harari³, George Coukos, Olaia Naveiras⁴, Nicola Vannini*¹

¹Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne,University of Lausanne, ²Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences,Swiss Federal Institute of Technology Lausanne (EPFL), ³Center of Experimental Therapeutics, Department of Oncology,Centre Hospitalier Universitaire Vaudois, ⁴Hematology Service, Department of Oncology,Centre Hospitalier Universitaire Vaudois (CHUV)

Chapters

00:04Title
01:59Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
04:00Measurement of Mitochondrial Mass and Membrane Potential
06:21Results: Mitochondrial Profiles
07:18Conclusion

Summary

자동 번역

在这里，我们描述了一种可靠的方法来测量线粒体质量和膜电位在体外培养造血干细胞和T细胞。

Tags

Keywords: Mitochondrial Mass Mitochondrial Membrane Potential Flow Cytometry Hematopoietic Stem Cells T-cells Metabolic Assays Bone Marrow Transplantation Colony-forming Assays Immune T Cells Verapamil FACS Sorting LKS Population CD150 CD48 Stem Cell Expansion Medium

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