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使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
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使用IBA1和TMEM119进行小鼠脑微胶质密度、形态学和周围骨髓细胞渗透分析的免疫荧光染色

DOI:
10.3791/60510-v

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10:40 min

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October 27, 2019

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, , , , ,
1Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, 2Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, 3Integrated Program in Neuroscience,McGill University, 4Center for Brain Immunology and Glia (BIG),University of Virginia

Chapters

  • 00:04Title
  • 01:00Density and Distribution Analysis
  • 05:50Morphology Analysis
  • 09:01Results: Immunofluorescence Staining Using IBA1 and TMEM119
  • 10:06Conclusion

Summary

자동 번역

자동 번역

该协议描述了IBA1和TMEM119免疫荧光结化的分步工作流程，除了分析微胶质密度、分布和形态，以及小鼠脑组织中外周髓细胞渗透。

Tags

ImmunofluorescenceIBA1TMEM119MicrogliaPeripheral Myeloid CellDensityMorphologyInfiltrationMouse BrainFijiImageJNearest Neighbor DistanceScaleComposite ImageChannel ToolFreehand SelectionSelection BrushRegion Of Interest

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