Journal
/
신경과학
/
遗传性痉挛性截瘫中人类诱导多能干细胞衍生神经元的线粒体迁移与形态分析
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia
Please note that all translations are automatically generated. Click here for the English version.

遗传性痉挛性截瘫中人类诱导多能干细胞衍生神经元的线粒体迁移与形态分析

DOI:
10.3791/60548-v

•

07:32 min

•

February 09, 2020

•

, , , ,
1Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, 2Department of Bioengineering,University of Illinois at Chicago, 3MD Program,University of Illinois College of Medicine Rockford

Chapters

  • 00:04Introduction
  • 00:50Telencephalic Glutamatergic Neuron Generation
  • 01:49Time-Lapse Imaging of Mitochondrial Transport Along Telencephalic Glutamatergic Neuron Axons
  • 02:44Cortical Neuron Mitochondrial Transport Analysis
  • 04:17Cortical Neuron Mitochondrial Morphology Analysis
  • 05:32Results: Representative Mitochondrial Transport and Morphology Analyses
  • 06:47Conclusion

Summary

자동 번역

자동 번역

受损的线粒体迁移和形态学涉及各种神经退行性疾病。提出的协议使用诱导多能干细胞衍生前脑神经元来评估线粒体迁移和遗传性痉挛性截瘫的形态。该协议允许沿斧子对线粒体贩运进行表征，并分析其形态，这将有助于神经退行性疾病的研究。

Tags

Mitochondrial TransportMitochondrial MorphologyHuman Induced Pluripotent Stem Cell-derived NeuronsHereditary Spastic ParaplegiaAxonal DegenerationMitochondrial DynamicsNeurological DiseasesLive Cell ImagingMitochondrial TraffickingTherapeutic TargetsNeurodegenerative DiseasesNeurosphere DifferentiationAxon IdentificationMitochondrial StainingTime-lapse ImagingImageJ Analysis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

神经调节和线粒体运输：在较长的时间里，海马神经元的实时成像

人类神经祖细胞和神经元小分子诱导人类胚胎干细胞的多能干高效的推导

从人类多能干细胞的规范在前脑谷氨酸能神经元

分析树突状形态的列和图层

利用干细胞衍生脑 Organoids体外培养人脑组织 Zika 病毒感染的模拟研究

96井格式人诱导多潜能干细胞源神经元高速拉伸损伤的方法

预组装塑料微流体芯片中人类干细胞衍生神经元的分段化

分化后再蛋白的人类多能干细胞衍生神经元，用于高含量的内尿成生和突合性成熟筛查

利用 PiggyBac 载体将人诱导的多能干细胞 (Ipsc) 转化为功能性脊柱和颅内运动神经元

人类干细胞衍生内皮细胞和GABAergic神经元的迁移、化学吸引和共培养测定

Read Article