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Chapters
- 00:04Introduction
- 00:39S. aureus Preparation
- 01:57Mouse Implant Placement and Inoculation
- 04:09Longitudinal In Vivo Bioluminescence Imaging (BLI)
- 04:46Implant‐Adherent and Surrounding Tissue Bacteria Quantification
- 06:16Results: Representative Bacterial Burden Quantification and Analysis
- 07:30Conclusion
The protocol describes a novel in vivo mouse model of spinal implant infection where a stainless-steel k-wire implant is infected with bioluminescent Staphylococcus aureus Xen36. Bacterial burden is monitored longitudinally with bioluminescent imaging and confirmed with colony forming unit counts after euthanasia.
