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Het combineren van fluidic devices met microscopie en flow cytometrie om microbiële transporten te bestuderen in poreuze media over ruimtelijke schalen
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JoVE 신문 환경과학
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
DOI:

12:32 min

November 25, 2020

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Chapters

  • 00:04Introduction
  • 00:57Preparation of A Microfluidic Device in Polydimethylsiloxane (PDMS)
  • 03:27Preparation of a Fluidic Device in Poly(methyl methacrylate)(PMMA)
  • 04:09Analyze Bacterial Transport using PDMS Microfluidic Devices
  • 05:10Basic Image Processing
  • 06:59Analyze Bacterial Transport at the Pore Scale
  • 08:08Study Bacterial Filtration by Means of Deposition Profiles
  • 08:51Analyze Bacterial Transport using PMMA Fluidic Devices
  • 10:33Results: Motile and Nonmotile GFP-tagged P. putida KT2440
  • 11:48Conclusion

Summary

자동 번역

Baanbrekende krommen (BBC's) zijn efficiënte instrumenten om het transport van bacteriën in poreuze media te bestuderen. Hier introduceren we tools op basis van vloeibare apparaten in combinatie met microscopie en flow cytometrische tellingen om BTC's te verkrijgen.

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