Journal
/
생물학
/
使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer
Please note that all translations are automatically generated. Click here for the English version.

使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数

DOI:
10.3791/61002-v

•

07:55 min

•

May 07, 2020

•

, , , ,
1Department of Physiology,Amsterdam UMC, 2Department of Kinesiology and Physical Education, Faculty of Education,McGill University, 3IONOptix BV

Chapters

  • 00:04Introduction
  • 00:50Myofibril and Mounting Needle Preparation
  • 02:08Experiment Initialization
  • 04:03Tension Redevelopment Rate Measurement
  • 05:04Stepwise Stretch
  • 05:33Results: Representative Active and Passive Tension Analyses
  • 07:03Conclusion

Summary

자동 번역

자동 번역

此处介绍的是一个协议，用于评估具有纳米-牛顿分辨率的条纹肌肉肌异半孔的收缩特性。该协议采用基于干涉测量的光学力探头的设置。此设置生成具有高信噪比的数据，并可评估 myofibril 的收缩动力学。

Tags

MyofibrilsSkeletal Muscle BiopsiesContractile FunctionNano-Newton Resolution Force TransducerSarcomere DysfunctionSarcomeric ProteinsPermeabilized CardiomyocytesHomogenizationMyofibril SuspensionRelaxing SolutionPoly-HEMA Coated SlideShellac Ethanol GlueMounting NeedleMicro-manipulatorsSarcomere LengthVessel FunctionVideo ImagingMicroscope Stage

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离

骨骼肌的线粒体分离

纯化的骨骼肌的先觉者

对大鼠的过程<em在原位</em骨骼肌收缩性能

模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞

单个肌纤维和成人骨骼肌卫星细胞的分离和培养

在收缩力测量评估肌肉功能在斑马鱼幼虫

骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动

肌肉活检中的免疫标记肌纤维退化

骨骼肌成纤维细胞外泌体的分离和表征

Read Article