Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
 

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Article DOI: 10.3791/61039-v 11:40 min April 17th, 2020
April 17th, 2020

챕터

요약

Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter proteins.

Tags

Reverse Genetics Recombinant Rotaviruses Reporter Proteins Fluorescent Marker Proteins BHKT7 Cells Plasmids Transfection Serum Medium Cell Culture Plate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter