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1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics,Medical University of Vienna (MUV), 2Murdoch Children’s Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health,Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry,Medical University of Vienna
Chapters
- 00:04Introduction
- 00:35Preparing Solutions and Plates (Day 1)
- 01:50Blocking and Sample Addition (Day 2)
- 03:42Antibody Addition
- 05:17Reading the Plate
- 06:10Results: Accuracy and Consistency of MeCP2-ECLIA
- 07:20Conclusion
Der Elektrochemilumineszenz-Immunoassay (ECLIA) ist ein neuartiger Ansatz für den quantitativen Nachweis endogener und exogen angewendeter MeCP2-Proteinvarianten, der hochquantitative, genaue und reproduzierbare Messungen mit geringem Intra- und Inter-Assay-Fehler über einen weiten Arbeitsbereich erzeugt. Hier wird das Protokoll für die MeCP2-ECLIA im 96-Well-Format beschrieben.
