JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:42Short Tandem Target Mimic (STTM) Fragment Amplification
- 01:40STTM Purification
- 02:22T4 DNA Polymerase Reaction Mix Preparation
- 03:13Potato Virus X (PVX)-Based microRNA (miRNA) Silencing (VbMS) Vector Construction
- 04:55T4 DNA Polymerase Reaction Setup and STTM Cloning
- 07:21Potato Virus-X-Based VbMS Assay
- 10:07Results: Representative VbMS of miR165/166 in Tetraploid Potato Species
- 11:16Conclusion
