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切除小鼠神经神经纤维神经丝的成像与分析

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Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

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切除小鼠神经神经纤维神经丝的成像与分析

DOI:

10.3791/61264-v

•

09:52 min

•

August 31, 2020

•

Nicholas P. Boyer, Maite Azcorra², Peter Jung⁴, Anthony Brown

¹Department of Neuroscience,The Ohio State University, ²Present address: Interdepartmental Neuroscience Graduate Program and Department of Neurobiology,Northwestern University, ³Quantitative Biology Institute,Ohio University, ⁴Department of Physics and Astronomy,Ohio University

Chapters

00:04Introduction
00:41Initial Assembly of Nerve Perfusion Chamber
01:26Dissection and Preparation of Mouse Tibial Nerve
03:09Final Nerve Perfusion Chamber Assembly
04:48Fluorescence Activation and Image Acquisition
06:21Flatfield and Darkfield Image Acquisition
07:37Results: Pulse-escape and Pulse-spread Experiments
09:17Conclusion

Summary

자동 번역

我们描述了荧光活化方法，用于分析来自转基因小鼠的周围神经单髓轴的轴感传输，这些轴感来自转基因小鼠，这些轴线来自表达可光激活神经丝蛋白的外周神经。

Tags

Neurofilament Transport Fluorescence Microscopy Photoactivatable Fluorescent Fusion Myelinated Axons Tibial Nerve Nerve Dissection Perfusion Chamber Oxygenated Saline Microdissection Nerve Imaging

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